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Code chicken core 1.0.4 plus#
Obtain superior images with the new Invitrogen™ Alexa Fluor™ Plus secondary antibodies. This antibody does not bind non-immunoglobulin mouse serum proteins or IgG from bovine, chicken, goat, guinea pig, hamster, horse, human, rabbit, rat or sheep. Specificity: This antibody binds to heavy chains on mouse IgG and light chains on all mouse immunoglobulins. Because staining protocols vary with application, the appropriate dilution of antibody should be determined empirically. This step will help eliminate any protein aggregates that may have formed during storage, thereby reducing nonspecific background staining. Using conjugate solutions: Centrifuge the protein conjugate solution briefly in a microcentrifuge before use add only the supernatant to the experiment. The benefits of these extra steps are apparent in multiplexing/multicolor-staining experiments where there is potential cross-reactivity with other primary antibodies or in tissue/cell fluorescent staining experiments where there may be the presence of endogenous immunoglobulins. Further passages through additional columns result in highly cross-adsorbed preparations of secondary antibody. Only the nonspecific-binding secondary antibodies are captured in the column, and the highly specific secondaries flow through. The secondary antibody solution is passed through a column matrix containing immobilized serum proteins from potentially cross-reactive species. Cross-adsorption or pre-adsorption is a purification step to increase specificity of the antibody resulting in less background staining and cross-reactivity. To minimize cross-reactivity, the donkey anti-mouse IgG whole antibodies have been cross-adsorbed against IgG from bovine, goat, chicken, guinea pig, hamster, horse, sheep, rabbit, rat, and human.